Leveraging Human Insights by Combining Multi-Objective Optimization with Interactive Evolution

Deceptive fitness landscapes are a growing concern for evolutionary computation. Recent work has shown that combining human insights with short-term evolution has a synergistic effect that accelerates the discovery of solutions. While humans provide rich insights, they fatigue easily. Previous work reduced the number of human evaluations by evolving a diverse set of candidates via intermittent searches for novelty. While successful at evolving solutions for a deceptive maze domain, this approach lacks the ability to measure what the human evaluator identifies as important. The key insight here is that multi-objective evolutionary algorithms foster diversity, serving as a surrogate for novelty, while measuring user preferences. This approach, called Pareto Optimality-Assisted Interactive Evolutionary Computation (POA-IEC), allows users to identify candidates that they feel are promising. Experimental results reveal that POA-IEC finds solutions in fewer evaluations than previous approaches, and that the non-dominated set is significantly more novel than the dominated set. In this way, POA-IEC simultaneously leverages human insights while quantifying their preferences

Molecular marks for epigenetic identification of developmental and cancer stem cells

Epigenetic regulations of genes by reversible methylation of DNA (at the carbon-5 of cytosine) and numerous reversible modifications of histones play important roles in normal physiology and development, and epigenetic deregulations are associated with developmental disorders and various disease states, including cancer. Stem cells have the capacity to self-renew indefinitely. Similar to stem cells, some malignant cells have the capacity to divide indefinitely and are referred to as cancer stem cells. In recent times, direct correlation between epigenetic modifications and reprogramming of stem cell and cancer stem cell is emerging. Major discoveries were made with investigations on reprogramming gene products, also known as master regulators of totipotency and inducer of pluoripotency, namely, OCT4, NANOG, cMYC, SOX2, Klf4, and LIN28. The challenge to induce pluripotency is the insertion of four reprogramming genes (Oct4, Sox2, Klf4, and c-Myc) into the genome. There are always risks of silencing of these genes by epigenetic modifications in the host cells, particularly, when introduced through retroviral techniques. In this contribution, we will discuss some of the major discoveries on epigenetic modifications within the chromatin of various genes associated with cancer progression and cancer stem cells in comparison to normal development of stem cell. These modifications may be considered as molecular signatures for predicting disorders of development and for identifying disease states